Description of the technology offer
The fluorescence holographic microscope provides dual image reconstructed from a hologram captured by a digital camera.
The first image visualizes optical section of the intensity of fluorescence radiation. The full-field optical section is obtained from a single hologram captured in one-shot, therefore no scanning across the field of view is needed and a high-speed imaging is possible. Optical sectioning effect may be stronger (enhanced axial resolution) compared to a standard confocal microscopy and the thickness of the optical section is constant across the field of view (unlike light-sheet microscopy).
The second image reveals the phase differences between the opposite sides of the sample based on the plane of focus and can be used to reconstruct the spatial distribution of refractive index within the sample. This is not possible to achieve with classical holographic microscopy, where the integral value of refractive index along the optical axis is obtained for each pixel. Spatial distribution of refractive index is a quantitative information and it is proportional to dry mass density for example of a sample like alive cell.
Optical sectioning of the intensity of fluorescence radiation showing the spatial distribution of naturally present substances or external dyes capable of emitting characteristic radiation combined with the spatial distribution of mass within the sample is a unique combination of the two visualisation modes and allows to study simultaneously the function and structure of living microobjects and eventually also time development of their reactions to various external stimuli (environment change, the presence of substances, drug application, …).
Main advantages
- Two image modes: spatial distribution of fluorochromes, quantitative reconstruction of spatial distribution of refractive index
- No lateral scanning needed, full-field of view by one-shot, high-speed imaging possible
- Live micro-objects imaging
Protection of intellectual property
- CZ utility model granted
- CZ patent applied for
- PCT will be applied
Offer of collaboration
We are offering a licence and an opportunity for contractual research collaboration.
Kontakt
Ing. Jana Ondroušková, Technology Transfer Office, This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it., +420 541 144 225
